On the first run of AutoConnect the database file TPACGlobal.db is created automatically (also whenever TPACGlobal.db was deleted). AutoConnect uses the registry values for the AutoConnect tables Map Additional Printers and Dynamic Printer Matrix (from local configuration and policies) to initialize the database.
Under normal circumstances (that is, the database is not deleted) the AutoConnect tables Map Additional Printers and Dynamic Printer Matrix will be imported just once. Customers who update the old registry values using the old GPOs will not see these changes. The old GPOs should be removed and the new GPOs should be set up corresponding the Group Policies documentation.